Identification of mobile inhibitors in opposition to Chikungunya virus replication

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Identification of cellular inhibitors in opposition to Chikungunya virus replication by a cDNA expression cloning combined with MinION sequencing

  • cDNA expression cloning has been confirmed to be a robust technique inside the search for cellular parts that administration virus replication. On this study, cDNA library screening using a pool of cDNA derived from interferon-treated human cells was combined with the MinION sequencer to determine cellular genes inhibiting Chikungunya virus (CHIKV) replication.
  • Downside an an infection of CHIKV to Vero cells transduced with the cDNA library produced virus-resistant cells. Then, the MinION sequence of cDNAs extracted from the surviving cells revealed that the open finding out frames of TOM7, S100A16, N-terminally truncated kind of ECI1 (ECI1ΔN59), and RPL29 have been inserted in a number of the cells.
  • Importantly, the transient expression of TOM7, S100A16, and ECI1ΔN59 was found to inhibit the replication of CHIKV in Huh7 cells, indicating that these cellular parts have been doubtlessly anti-CHIKV molecules.
  • Thus, our study demonstrated that cDNA expression cloning combined with the MinION sequencer allowed a quick and full detection of cellular inhibitors in opposition to CHIKV.
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XPO6 Antibody

1-CSB-PA026224GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity Purified
Description: A polyclonal antibody against XPO6. Recognizes XPO6 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC

XPO6 siRNA

20-abx940017
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

XPO6 siRNA

20-abx940018
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

XPO6 Rabbit pAb

A10401-100ul 100 ul
EUR 308

XPO6 Rabbit pAb

A10401-200ul 200 ul
EUR 459

XPO6 Rabbit pAb

A10401-20ul 20 ul
EUR 183

XPO6 Rabbit pAb

A10401-50ul 50 ul
EUR 223

XPO6 Blocking Peptide

DF12799-BP 1mg
EUR 195

XPO6 Conjugated Antibody

C46712 100ul
EUR 397

XPO6 cloning plasmid

CSB-CL842747HU1-10ug 10ug
EUR 814
  • Formulation: 10 μg plasmid + 200μl Glycerol
  • Length: 2514
  • Sequence: atggcgtcagttaacggcagcagccagaactgtgtctcgggtcaggagcgcggccggctgggggtcctggccatgtcctgcatcaatgaactcatgtccaagaactgtgtgcctatggaatttgaggagtatttactgcgtatgttccagcagactttctacctcctgcagaaaa
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Description: A cloning plasmid for the XPO6 gene.

XPO6 cloning plasmid

CSB-CL842747HU2-10ug 10ug
EUR 1202
  • Formulation: 10 μg plasmid + 200μl Glycerol
  • Length: 3378
  • Sequence: atggcatctgaagaagcctctctcagggcattggaaagtctgatgacagaattttttcacgattgtacaaccaatgaaagaaaacgtgagatagaggagcttcttaataactttgcccagcaaataggagcctggagattctgcctgtactttctctccagcactaggaatgact
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Description: A cloning plasmid for the XPO6 gene.

anti- XPO6 antibody

FNab09550 100µg
EUR 505.25
  • Recommended dilution: IHC: 1:10-1:100
  • IP: 1:200-1:1000
  • Immunogen: exportin 6
  • Uniprot ID: Q96QU8
  • Gene ID: 23214
  • Research Area: Signal Transduction
Description: Antibody raised against XPO6

Anti-XPO6 antibody

PAab09550 100 ug
EUR 355

Anti-XPO6 antibody

STJ112437 100 µl
EUR 277
Description: The protein encoded by this gene is a member of the importin-beta family. Members of this family are regulated by the GTPase Ran to mediate transport of cargo across the nuclear envelope. This protein has been shown to mediate nuclear export of profilin-actin complexes. A pseudogene of this gene is located on the long arm of chromosome 14. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Anti-XPO6 antibody

STJ13100475 100 µl
EUR 427

Exportin 6 (XPO6) Antibody

20-abx112420
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
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Exportin-6 (XPO6) Antibody

20-abx126798
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Exportin-6 (XPO6) Antibody

abx037309-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Exportin-6 (XPO6) Antibody

abx239550-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

XPO6 ELISA KIT|Human

EF004323 96 Tests
EUR 689

Mouse XPO6 shRNA Plasmid

20-abx978151
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Exportin-6 (XPO6) Antibody

20-abx321354
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Human XPO6 shRNA Plasmid

20-abx958092
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human Exportin 6 (XPO6) Protein

20-abx650936
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Xpo6 ORF Vector (Mouse) (pORF)

ORF061968 1.0 ug DNA
EUR 506

Xpo6 ORF Vector (Rat) (pORF)

ORF079217 1.0 ug DNA
EUR 506

XPO6 ORF Vector (Human) (pORF)

ORF014966 1.0 ug DNA
EUR 354

XPO6 ORF Vector (Human) (pORF)

ORF011649 1.0 ug DNA
EUR 95

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
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Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Human Exportin- 6, XPO6 ELISA KIT

ELI-17963h 96 Tests
EUR 824

Mouse Exportin- 6, Xpo6 ELISA KIT

ELI-51533m 96 Tests
EUR 865

Human Exportin-6 (XPO6) ELISA Kit

abx384332-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Xpo6 sgRNA CRISPR Lentivector set (Mouse)

K4963501 3 x 1.0 ug
EUR 339

Xpo6 sgRNA CRISPR Lentivector set (Rat)

K6323501 3 x 1.0 ug
EUR 339

XPO6 sgRNA CRISPR Lentivector set (Human)

K2650901 3 x 1.0 ug
EUR 339

Human Exportin 6(XPO6)ELISA Kit

QY-E01489 96T
EUR 361

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
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cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

Xpo6 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4963502 1.0 ug DNA
EUR 154

Xpo6 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4963503 1.0 ug DNA
EUR 154

Xpo6 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4963504 1.0 ug DNA
EUR 154

Xpo6 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6323502 1.0 ug DNA
EUR 154

Xpo6 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6323503 1.0 ug DNA
EUR 154

Xpo6 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6323504 1.0 ug DNA
EUR 154

XPO6 sgRNA CRISPR Lentivector (Human) (Target 1)

K2650902 1.0 ug DNA
EUR 154

XPO6 sgRNA CRISPR Lentivector (Human) (Target 2)

K2650903 1.0 ug DNA
EUR 154

XPO6 sgRNA CRISPR Lentivector (Human) (Target 3)

K2650904 1.0 ug DNA
EUR 154

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415
 

Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing

Purpose: To report on a novel KIR3DL3 allele acknowledged in a southern Han Chinese language language explicit particular person.

Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive consequence by KIR3DL3 sequence-based typing (SBT). Entire mRNA was extracted and subjected to reverse transcription to accumulate KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.

Outcomes: cDNA cloning and sequencing have acknowledged a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been formally assigned by the KIR subcommittee of World Effectively being Group Nomenclature Committee for parts of HLA system.

Conclusion: cDNA cloning and sequencing may be used to inform aside inconclusive ends in KIR3DL3 SBT with a objective to determine novel KIR alleles. 

Apple Russet Ring and Apple Inexperienced Crinkle Sicknesses: Achievement of Koch’s Postulates by Virome Analysis, Amplification of Full-Measurement cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Duplicate of Indicators on Fruits of Apple Bushes Inoculated With Viral RNAs

Apple russet ring and apple inexperienced crinkle are graft-transmitted diseases first reported higher than 60 years previously, nevertheless at present, no affiliation between a specific virus (variant) and the sickness has been clearly demonstrated.

 

On this study, we carried out the following assortment of experiments to determine the causal viruses (variants) of these apple diseases; (1) full analysis by next-generation sequencing of all viruses in each apple tree affected with russet ring or inexperienced crinkle sickness,

 

(2) amplification of full-length genomic cDNA of viruses using primers containing the T3 promoter and the in vitro transcription of infectious viral RNAs, (3) inoculation of viral RNA transcripts to every herbaceous and apple crop, (4) analysis of sequence variants of viruses present in contaminated crops.

(5) back-inoculation of sequence variants of candidate viruses to apple seedlings combined with the virus-induced flowering know-how using the apple latent spherical virus vector to breed the symptom on the fruit as shortly as doable, and (6) reproduction of indicators on the fruits of apple timber inoculated with sequence variants and the re-isolation of each virus variant from apples exhibiting fruit indicators.

The outcomes confirmed that one in every of many sequence variants of the apple chlorotic leaf spot virus causes a attribute ring-shaped rust on the fruits of contaminated apple timber and {{that a}} sequence variant of the apple stem pitting virus possibly causes inexperienced crinkle indicators on an contaminated apple fruit.

Thus, we’ve got been able to fulfill Koch’s postulates to point out the viral etiology of every the apple russet ring and inexperienced crinkle diseases. We moreover counsel an experimental system which will present whether or not or not a virus current in diseased tissues is the pathogen accountable for the diseases when the etiology is undetermined.

Antidiabetic and In VitroEnzyme Inhibition Analysis of Methanol Extract of Ocimumtenuiflorum Linn Leaves and Its Fractions

The current analysis aimed to seek out out the easiest dose of methanol extract of Ocimumtenuiflorum L. leaves extract, and it is a fraction to blood-glucose-lowering in diabetic rats, and evaluated the α-amylase, α-glucosidase inhibitors and insulin diploma of diabetic rats used to realize bigger administration over hyperglycemia.

The outcomes of the antihyperglycaemic of oral administration of a particular dose of methanol extract in streptozotocin-induced rats confirmed that the perfect dose of methanol extract significantly diminished the blood glucose diploma as compared with one different dose.

Moreover, the outcomes of repeated administration of methanol fractions signifies that ethyl acetate-butanol fraction exhibited a stronger antihyperglycemic impression than chloroform and ethanol-water fractions. Moreover, the result confirmed that impression of methanol extract and its fraction on α-glucosidase and α-amylase enzymes actions and its insulin diploma by in vitro analysis, ethyl acetate-butanol fraction could administration with low focus as compared with completely different fractions and acarbose that used as a constructive administration.

From the outcomes of insulin diploma, methanol extract and fraction did not current any vital. These findings indicated that the energetic crude extract (methanol) and its energetic fractions (ethyl acetate/butanol) could exert vital glucose-lowering impression due to the presence of polyphenolics energetic constituents. In conclusion, isolation of the energetic parts of Ocimumtenuiflorum L. might pave the best way by which to the occasion of newest brokers for the remedy of diabetes and its points.

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